In tilt-pair data collection, each molecule is imaged twice at different but controlled views. The tilt-pair data can substantially simplify the analysis of angular reconstitution because particle orientations are now restrained by the experimental tilt-geometry. In a tilt-pair model reconstruction, corresponding particles from tilt-related micrographs are grouped into a pair of stacks, {A} and {B}. Then, particles in stack {A} are sorted into homogeneous classes. From one such class of {A}, a 3D model can be reconstructed directly by using the tilt-related particles in stack {B}.
The tilt-pair processing pipe-line implemented in PARTICLE is summarized in the following flow-chart:
